Ion exchange chromatography

Ion exchange chromatography (HPIC)

Analyte:

  • Inorganic and organic ions, carbohydrates, amino acids, proteins, peptides, transition metals, alkali and earth metals, ammonium, amines
  • Principle of reversible adsorption
  • Required sample amount: µL-range

 

Applications:

  • Identification of substance mixtures
  • Separation of substance mixtures into individual components by different electrical properties
  • Determination of substances / chromatographic separation depends on:
    • Charge / size of ions
    • Type of the counterions in mobile phase
    • pH-value in mobile phase
    • Concentration of  mobile phase
    • Type of  ion exchanger

 

Technical equipment:

ICS-5000 (Co. Dionex)

  • Pump: DP quaternary gradient pump ICS5+
  • Flow rate: 0.001 – 10 mL / min
  • Detector: CD conductivity detector (analytical), ED amperometric detector for capillary and analytical application
  • Autosampler: injection volume 0.4 µL – 7.5 mL, automatic dilution (1:1 up to 1 : 1,000), temperature range 4 – 60 °C